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Concentrations in published research range from 0.05% to 1%, depending on the target tissue and intended outcome

They are reference points for researchers designing their own evidence-based protocols

In vitro research has demonstrated that hCG decreases TIMP-1 secretion in endometrial stromal cells, thereby facilitating extravillous trophoblast invasion with MMP-2 levels increasing and gelatine zymography confirming increased MMP-2 activity following hCG treatment

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Second, any mild stimulatory effects from improved cellular energy production are more appropriate during waking hours than before bedtime