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Initially, genomic DNA (50200 ng) was processed and converted with the Zymo EZ DNA Methylation Kit (Zymo Research, Irvine, CA, USA), followed by multiple rounds of bisulfite PCR amplification (approximately 2533 cycles) for targeted amplification

Durn RV, MacKenzie ED, Boulahbel H, Frezza C, Heiserich L, Tardito S, Bussolati O, Rocha S, Hall MN, Gottlieb E

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Proneuronal GBM cancer stem cell lines were reported to downregulate NKG2D expression on NK cells through transforming growth factor-beta-dependent suppression, providing an explanation for the reduced immune infiltration [17]

Pyun J, McInnes LE, Donnelly PS et al (2022) Copper bis(thiosemicarbazone) complexes modulate P-glycoprotein expression and function in human brain microvascular endothelial cells