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On the one hand, the vaccination population in the small to medium scale studies we included were mostly composed by health care workers, patients with specific diseases or the elderly

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The shots are most effective for individuals with a vitamin B12 deficiency

If your protocol requires you to store it for many months after reconstitution, a better (though more advanced) method is to aliquot itdivide the full solution into multiple smaller sterile tubes and freeze them all at once