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An interaction term was also included in the model as shown below: DESeqDataSet(group1_vs_group2,design = ~ X1 + X2 + X3 + X4 + X5 + X6 + X7 + X8 + X9 + X10 + Stimulus + Hours + Stimulus:Hours)

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1 To date, multiple mechanisms of tissue damage have been implicated in SLE, including oxidative stress and free radicals, fibrosis, complement-mediated pathways, and a panoply of enzymatic cascades including matrix metalloproteinases, the renin-angiotensin system, plasmin, and kallikreins