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Although statistical significance was not observed in the Liverpool replication cohort because of its small sample size, the direction of effect was consistent with the primary analysis

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May increase dose gradually

It restores the seal between gut lining cells (tight-junction proteins), heals the intestinal lining, and reduces mucosal inflammation

A 24 h time point was chosen based on a previous report of using this time period for transfecting or treating 3T3-L1 cells with NNMT anti-sense oligonucleotides or a small molecule NNMT product inhibitor (1-MNA), respectively, for phenotypic measures