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Lysates were clarified by centrifugation at 12,000 g for 30 minutes at 4 C and the supernatant removed

Science 346 , 318322 (2014)

Moreover, iron is part of the prosthetic group of catalases, key antioxidant enzymes that counteract oxidative stress by catalyzing the reduction and detoxification of hydrogen peroxide

World Allergy Organ J 5:919 Chen HG et al (2013) Heme oxygenase-1 mediates the anti-inflammatory effect of molecular hydrogen in LPS-stimulated RAW 264.7 macrophages