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Reactions consisted of 10 L assay buffer (50 mM HEPES, 50 mM NaCl, various pH), 10 L enzyme (various concentrations), and 30 L DCF-G (400 M final) diluted in assay buffer

Research trials often employ a once-weekly subcutaneous micro-dosing approach, typically around 0.1 mL, to maintain consistent exposure levels in peptide models

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1 Introduction Cancer remains a leading cause of mortality around the world, with an estimated 19.3 million new cases and 10 million deaths from cancer in 2020 (Ferlay et al., 2021)