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The reaction was carried out in 10l reaction volume containing 25 ng of RNA, 0.25 M of each primer, 0.125 l iScript Reverse Transcriptase enzyme (BIO-RAD, Hercules, CA, USA), and 5 l of 2x one-step SYBR Green Reaction mix (BIO-RAD, Hercules, CA, USA)

Thus, exclusive glycolytic metabolism of the 60 nmol/mg glucosyl residues mobilized from glycogen to lactate [128] during 20 min starvation would regenerate 180 nmol/mg ATP, thereby having then potential to fuel 6 times a complete regeneration of the specific cellular ATP pool (around 30 nmol/mg) of cultured astrocytes and to supply the basal ATP consumption (4 nmol/(min x mg)) for 45 min

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