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The EMBO Journal

Macro- ATG consists in the delivery of cytoplasmic cargo to the lysosome through the intermediary of a double membrane bound vesicle, the autophagosome, which fuses with the lysosome to form an autolysosome ( Accumulating data point to an essential role for ROS as mediators in the activation of ATG under several stimuli (starvation, pathogens, death receptors), even though the nature of this involvement remains still elusive

Cells were extracted with 200 L of ice-cold 80% methanol, centrifuged at 15,000 g for 20 min at 4C and lyophilized

This is accomplished by inserting the GST DNA coding sequence next to that which codes for the protein of interest

Hepatology (2014) 61:98107