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The kinetic parameters ( k cat and apparent K m ) and the inhibition constants ( K i ) were, respectively, obtained by fitting the data to the nonlinear regression MichaelisMenten model (kinetic assays) and to the mixed inhibition model (inhibition assays) in GraphPad Prism 8 software ( k cat values were expressed as mol of substrate oxidized per second per mol of enzyme ( i.e ., the turnover number in s 1 ), using specific molar absorption coefficients of 9600 M 1 cm 1 at 340 nm for CDNB, 9250 M 1 cm 1 at 274 nm for BITC, 8890 M 1 cm 1 at 274 nm for PITC, 17,700 M 1 cm 1 at 412 nm for PNP-butyrate, 13,750 M 1 cm 1 at 224 nm for HNE, and 6220 M 1 cm 1 at 340 nm for NADPH

As mitochondria produce energy, they naturally generate free radicals as a byproduct of metabolism

SZOT, R.J

Acta Biochimica et Biophysica Sinica, 46(10), 902907

Will continue to use a few more months hoping to see a positive change